Raafat Awadallah, Ehsan Aly Badawy, Maha Elwassef, Mehrevan Abd El-Moniem, Eman R Youness and Mona Anwar, National Research Center, Egypt
Abstract: Generation of free radical with subsequent oxidative stress has been involved in biochemical and molecular mechanisms of Cadmium (Cd) toxicity. The aim of this study was to investigate the nutraceutical effects of diallyltetrasulfide (DTS), (an organosulfer compound derived from garlic) on Cd induced renal toxicity in rats. Renal toxicity was induced by subcutaneous injection of Cd chloride (3mg/kg/bw/day). Forty Albino rats were used in this study; the first group was injected subcutaneously with isotonic saline and received intragastric corn oil. The second group received intragastric DTS 40 mg/Kg/bw/day. The third group was injected with the same dose of Cd chloride. The forth group was injected with Cd chloride followed by intragastric DTS. At the end of the experiment (30 days) urine samples were collected, rats were sacrificed and blood samples (4ml) were collected for biochemical analysis. Significant increased levels of urea, creatinine, advanced oxidative protein products and 8-hydroxy-2’-deoxyguanosine (p < 0.001) as well as significant decreased levels of serum total protein and antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were observed in the third group (Cd intoxicated rats) (p < 0.001). Significant decreased (p< 0.01) levels of urea, creatinine , advanced oxidative protein products and 8 hydroxyguanosine as well as significant increased levels (p< 0.01) of antioxidant enzymes in the forth group after intragastric administration of DTS . The data confirmed the nutraceutical property of 40 mg/Kg/bw/day DTS as an antioxidant that ameliorates oxidative stress and revealed that it is efficiently protects kidneys from Cd-induced oxidative damage.
Keywords: Cadmium; Diallyltetrasulfide; Antioxidant enzymes; Nutraceuticals; Renal toxicity; Oxidative stress